ERANET-LAC CarboxAID Project-Germany-Türkiye-Brazil-Ecvator

by Barış Darcan | Oct 20, 2023
The CarboxAID Project numbered 118Y270 "Valorisation of Agricultural Residues via Anaerobic Digestion: From Biogas to Carboxylates" was successfully completed with new patents. This project was realized with the participation of many countries and institutes. ERANet-LAC stands for European Union (EU), Latin America and the Caribbean Countries (LAC) on Joint Innovation and Research Activities, Germany, Türkiye, Brazil and Ecvador. For more information about the TUBITAK Eranet-LAC Multi-Cooperation project, please refer to the poster below.

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MEG GROUP

BOUN & ITU

ITU-BOUN MEG | Microbial Ecology Group

At the ITU-BOUN Microbial Ecology Group (MEG), we investigate the intricate roles of microorganisms in both engineered systems and natural environments through a multidisciplinary lens. With our experienced team and robust infrastructure, we conduct high-impact research that integrates microbial ecology, environmental biotechnology, molecular biology, and systems-based approaches to address complex ecological and environmental challenges.

What We Do

We conduct both fundamental and applied research to elucidate microbial interactions and functions across a broad spectrum of ecosystems—from engineered environments such as water and wastewater treatment and agro waste/solid waste/manure and sludge management, to natural and extreme habitats including air, water, sediments, hydrothermal systems, and polar permafrost zones.

Our Core Areas of Expertise Include:

  • Biotreatment and Bioremediation: Development of innovative microbial-based technologies for the treatment of solid, liquid, and gaseous wastes, and the remediation of hydrocarbon- and heavy metal-contaminated ecosystems.
  • Biomonitoring and Environmental Diagnostics: Application of high-resolution molecular tools (e.g., qPCR, metagenomics, amplicon sequencing, transcriptomics) for real-time monitoring and functional characterization of microbial communities in both environmental and engineered systems.
  • Bioenergy: Exploration and optimization of anaerobic digestion, biohydrogen, and biogas production processes through microbial process engineering and omics-based process enhancement.
  • Microbial Resource Exploration: Isolation, identification, and functional profiling of novel microbial taxa—including thermophiles, psychrophiles, halophiles, and extremophiles—from unique and polyextreme environments for biotechnological applications.

Field-Based Investigations

Our laboratory capabilities are complemented by comprehensive field research programs. Some of our recent and ongoing projects include:

  • Arctic Ecosystems: Investigations in Russia’s Arctic territories—particularly around the Tazovsky region—focused on the impacts of permafrost thaw, climate change, and land use shifts on microbial community dynamics, biogeochemical cycles, and greenhouse gas emissions.
  • Salda Lake (Türkiye): As one of the closest terrestrial analogs to Mars' Jezero Crater, Lake Salda offers a unique setting to study microbial life in high-alkaline, magnesium-rich, and ultra-oligotrophic conditions. We explore microbial adaptations, biomineralization processes, and potential biotechnological applications under these extreme conditions.
  • Marine Microbial Ecology: Biodiversity and function-based assessments in the Black Sea, Mediterranean, Aegean, and Marmara Seas, including anoxic sediments and petroleum-contaminated marine environments.
  • Urban Aquatic Systems (e.g., Golden Horn): Longitudinal studies on microbial community shifts in response to anthropogenic impacts, environmental restoration, and urban hydrological changes.
  • Extreme and Polyextreme Environments: Microbial surveys and metagenomic investigations in geothermal fields, deep-sea basins, cold-adapted high-altitude lakes, and other sites with combinations of physicochemical stressors.

Innovation through Molecular and Biotechnological Tools

Our group has developed several patented biotechnological methods and custom-engineered diagnostic platforms that enable:

  • Tailor-made environmental solutions for complex pollution problems,
  • Microbial community engineering for waste valorization and resource recovery,
  • Design of synthetic microbial consortia for climate-resilient bioprocesses.

We are also pioneers in applying integrated omics, synthetic biology, and AI-based modeling for the dynamic control and optimization of microbial systems. Our unique methodologies are applied across diverse sectors—from sanitation to environmental restoration and carbon sequestration.

Global Collaborations and Impact

Our group actively contributes to global-scale research consortia and mega projects, including the TerrArctic Project, where we provide microbial and environmental data to support climate adaptation strategies in Arctic and sub-Arctic regions.

Through our multidisciplinary research, engineering innovations, and field-based insights, we aim to bridge science and practice—delivering sustainable solutions for today’s most pressing environmental challenges.

 

Cutting-Edge Molecular Techniques and Technologies at MEG Laboratories

At MEG, we utilize a wide array of state-of-the-art molecular biology tools and technologies to investigate microbial communities, host-microbe interactions, and environmental microbiomes. Our infrastructure supports both fundamental research and applied biotechnology development. Below is an overview of the core techniques currently in use at our laboratories:


Next-Generation Sequencing (NGS) Platforms

We employ a broad range of high-throughput sequencing methods to explore genetic, transcriptomic, and epigenetic landscapes.

  • Whole Genome Sequencing (WGS):
    Comprehensive analysis enabling the detection of single nucleotide polymorphisms (SNPs), structural variations (insertions, deletions, inversions), copy number variations (CNVs), and rare mutations in microbial and host genomes.
  • Metagenomic Sequencing (Shotgun & Amplicon-Based):
    Culture-independent analysis of microbial diversity and function from environmental samples (e.g., soil, water, sludge). Both shotgun metagenomics and targeted amplicon sequencing (e.g., 16S/18S rRNA, ITS) are used.
  • Total RNA Sequencing (RNA-Seq):
    Analysis of transcriptomes to quantify gene expression, alternative splicing events, and non-coding RNA activity in microbial communities and host systems.
  • ChIP-Seq (Chromatin Immunoprecipitation Sequencing):
    Used in epigenomic studies to detect protein-DNA interactions, transcription factor binding sites, and histone modifications.
  • Targeted DNA Sequencing:
    Enables deep sequencing of specific genomic regions of interest (e.g., microbial virulence genes, ARGs, or cancer-associated genes) for high-sensitivity variant detection.
  • Methyl-Seq & ATAC-Seq:
    Applied for DNA methylation profiling and chromatin accessibility studies in host-microbe interaction models.

Third-Generation & Portable Sequencing Technologies

  • Nanopore Sequencing (Oxford Nanopore – MinION & MinIT):
    Real-time, long-read sequencing technology for field and lab-based applications including:
    • De novo genome assembly
    • Rapid diagnostics
    • Plasmid tracking
    • Mobile sequencing in remote sites
  • Miseq (Illumina):
    Short-read sequencing for amplicon, small genome, and targeted sequencing applications, often integrated with our metagenomic and microbiome workflows.

Conventional and Targeted Sequencing

  • Sanger Sequencing (Standard & Targeted):
    Applied for mutation screening, plasmid verification, and validation of NGS data. Still widely used for precision sequencing in specific gene targets.

Quantitative and Digital PCR-Based Technologies

  • Quantitative PCR (qPCR):
    Used for accurate quantification of microbial load, gene expression, and pathogen detection. Applied in environmental, clinical, and industrial microbiology.
  • Digital Droplet PCR (ddPCR):
    Advanced absolute quantification method providing superior precision and sensitivity, especially for low abundance targets and rare variant detection.

Complementary Molecular Tools

  • Microarray Technologies:
    Applied for high-throughput screening of gene expression or microbial community profiling in large sample cohorts.
  • CRISPR-Cas Systems:
    Used in functional gene studies, microbial strain editing, and diagnostic tool development.
  • Library Preparation Automation (e.g., NEBNext, Illumina DNA Prep):
    Enhances reproducibility and throughput in NGS workflows.

Message From The Coordinators

Orhan İnce

Prof. Dr. Orhan İnce

"At MEG, we continuously push the boundaries of microbial ecology by integrating cutting-edge molecular technologies with a multidisciplinary approach. Our strong infrastructure and collaborative spirit allow us to lead ambitious and transformative projects."





Bahar İnce

Prof. Dr. Bahar İnce

"Imagine a vibrant, research-driven group where every idea matters and where young scientists are empowered. At MEG, we cultivate innovation, excellence, and a sense of family — our roots are strong, and our future is bold."